Biochemistry and the Pasteur effect, backing please!?
In his studies of glucose metabolism by yeast, Louis Pasteur discovered that the sudden addition of oxygen (O2) to a previously anaerobic suspension of yeast in grape juice resulted contained by a dramatic decrease in the rate of glucose consumption. This “Pasteur effect” can be counteracted by the addition of 2,4-dinitrophenol (DNP), an uncoupler of the mitochondrial electron transport secure.
a) Why do yeast cells consume less glucose in the presence of oxygen? Can you estimate how much smaller amount glucose they use in the presence of oxygen?
b) Why does DNP counteract or prevent the Pasteur effect?
Answers:
Yeast cells require less glucose surrounded by the prescience of oxygen.
GOOD LUCK.
a) With the presence of oxygen (aerobic oxidation) glucose yields more ATP (look up how many moles) therefore yeast cell get more ATP (energy) from glucose under aerobic condition therefore consumes less glucose, while the conflicting for anerobic condition.
b)DNP blocks ATP production (look it up for verification) under aerobic conditions; therefore even under aerobic condition the yeast cell would consume more glucose to capture the same amount of ATP (when compared to without DNP present).
I bet you go to UT dallas!! I consistency your pain. Good luck Source(s): http://www.uta.edu/biology/badon/classnotes/3444/Lecture_15.htm
http://www.rebases.com/problem-sets.html
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a) Why do yeast cells consume less glucose in the presence of oxygen? Can you estimate how much smaller amount glucose they use in the presence of oxygen?
b) Why does DNP counteract or prevent the Pasteur effect?
Answers:
Yeast cells require less glucose surrounded by the prescience of oxygen.
GOOD LUCK.
a) With the presence of oxygen (aerobic oxidation) glucose yields more ATP (look up how many moles) therefore yeast cell get more ATP (energy) from glucose under aerobic condition therefore consumes less glucose, while the conflicting for anerobic condition.
b)DNP blocks ATP production (look it up for verification) under aerobic conditions; therefore even under aerobic condition the yeast cell would consume more glucose to capture the same amount of ATP (when compared to without DNP present).
I bet you go to UT dallas!! I consistency your pain. Good luck Source(s): http://www.uta.edu/biology/badon/classnotes/3444/Lecture_15.htm
http://www.rebases.com/problem-sets.html
Related Questions: