Pathogen Inactivation of blood components?
Hey guys, I've been researching on pathogen inactivation but haven't been able to fathom out why the chemicals added to blood don't inactivate the blood cells and only bind to pathogens. Can anyone provide a good explanation?
Thanks!
Answers:
Amotosalen, methylene blue and riboflavin adjectives inactivate pathogens by blocking replication of the nucleic acid genome of viruses, bacteria or vermin that may be present in donated blood. If a pathogen can't replicate, it can't infect the transfusion recipient.
The inactivation process doesn't compromise the function of the blood components because platelets and red blood cells are terminally differentiated cell (they don't replicate and don't have nuclear DNA) and plasma is just a liquid, not a cellular product. So nucleic acid-based inactivation processes are target something which is critical to the infectivity of pathogens, but not to the function of the blood component itself.
The donor's white blood cells may also be present in the donated blood. These cells do contain nucleus, and may be inactivated by the pathogen inactivation treatment (varies by method). However, the donor's white cells can cause complications to the recipient (transfusion-associated graft versus host disease, acute transfusion reactions), so it's truly desirable to inactivate white cells.
A solvent-detergent pathogen inactivation method is also available for plasma. Because this method's mechanism of action is to disrupt lipid membranes, it cannot be applied to platelets and red blood cell.
You can find out more about amotosalen and its mechanism of action on the product website:
http://www.interceptbloodsystem.com/mechanism_of_action.html
I'm sure the other manufacturer will also have information available online.
I hope you find this explanation useful.
Best regards,
Lainie
------------------------
Marketing Director
Cerus Corporation Source(s): http://www.interceptbloodsystem.com
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Thanks!
Answers:
Amotosalen, methylene blue and riboflavin adjectives inactivate pathogens by blocking replication of the nucleic acid genome of viruses, bacteria or vermin that may be present in donated blood. If a pathogen can't replicate, it can't infect the transfusion recipient.
The inactivation process doesn't compromise the function of the blood components because platelets and red blood cells are terminally differentiated cell (they don't replicate and don't have nuclear DNA) and plasma is just a liquid, not a cellular product. So nucleic acid-based inactivation processes are target something which is critical to the infectivity of pathogens, but not to the function of the blood component itself.
The donor's white blood cells may also be present in the donated blood. These cells do contain nucleus, and may be inactivated by the pathogen inactivation treatment (varies by method). However, the donor's white cells can cause complications to the recipient (transfusion-associated graft versus host disease, acute transfusion reactions), so it's truly desirable to inactivate white cells.
A solvent-detergent pathogen inactivation method is also available for plasma. Because this method's mechanism of action is to disrupt lipid membranes, it cannot be applied to platelets and red blood cell.
You can find out more about amotosalen and its mechanism of action on the product website:
http://www.interceptbloodsystem.com/mechanism_of_action.html
I'm sure the other manufacturer will also have information available online.
I hope you find this explanation useful.
Best regards,
Lainie
------------------------
Marketing Director
Cerus Corporation Source(s): http://www.interceptbloodsystem.com
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