What is staining affinity?

help me please.
Answers:
It is used in gram staining to differentiate gram negative and gram positive microbes. after being dyed, the cells are washed beside ethanol. gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram gloomy cells will not. iodine is used as a counter stain, which is up-taken by gram negative cells.

After the gram staining procedure is finished, gram positive cell will appear dark purple or blue due to the retained methylene blue. gram negative cells will appear pink or red due to the iodine counter stain.
The power of attraction of a stain towards a bacterial cell wall is call staining affinity. Source(s): http://answers.yahoo.com/question/index?…
http://en.wikipedia.org/wiki/Staining_(b…
http://wiki.answers.com/Q/What_is_staini…
This is the tendency for particular cells to pinch up certain dyes. The effect is utilised when looking at biological material to pick out certain cell,both normal and pathological. Source(s): GP for more years than I care to remember

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