How I multiply the concentration of a nucleic acerbic solution?
How I calculate the concentration of a nucleic acid solution from the optical density absorbance value measured by a spectrophotometer (using a wavelength of 260nm and 280nm contained by both solution TNE buffer or Distilled Water).
Answers:
You cannot do it directly. You need to make up two calibration standards, using known concentrations, against which you can compare spectrophotometric reading.
Actually, you can do it directly if you know the molar absorption coefficient or the extinction coefficient. Look up this value. This will tell you the absorbance of a 1 M solution. Since A is linear near concentration, you can do a calculation to obtain the concentration. This assumes that all the conditions are like as those used to get the extinction coefficient. If not, it should still give you a close approximation.
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Answers:
You cannot do it directly. You need to make up two calibration standards, using known concentrations, against which you can compare spectrophotometric reading.
Actually, you can do it directly if you know the molar absorption coefficient or the extinction coefficient. Look up this value. This will tell you the absorbance of a 1 M solution. Since A is linear near concentration, you can do a calculation to obtain the concentration. This assumes that all the conditions are like as those used to get the extinction coefficient. If not, it should still give you a close approximation.
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